Tissue Array Core Facility

UCLA Tissue Array Core Facility

Our Experience

Personnel

David Seligson, M.D. is the Director of the UCLA Tissue Array Core Facility, Co-Director of the Sarkaria Biomarker Innovation Laboratory and is a member of the Jonsson Comprehensive Cancer Center. He is an anatomical and clinical pathologist with post-doctoral fellowship training in both surgical pathology and medical genetics. Dr. Seligson founded the facility in 1999, creating one of the first tissue array core facilities in the world. He has extensive experience in the use of tissue array technology, particularly for tumor marker profiling studies.

Ms. Sheila Tze is the Laboratory Manager of the UCLA Tissue Array Core Facility and has been with the facility since its inception in 1999, providing pivotal support in the growth and development of the facility. She has eight years experience in tissue array project management, administration, design and construction. Ms. Tze is also the facility database manager and was instrumental in the creation of our “TMAtrix” database system.

Technical and support staff are fully trained to create tissue arrays of the highest quality and utility.

Facility

The core facility serves the general UCLA research community and has completed array construction and database projects for cancers of kidney, bladder, brain, prostate, breast, endometrium, lung and colon. In collaboration with UCLA neuropathologists, the facility was among the first to create arrays for specialized non-neoplastic diseases, such as Alzheimers Disease, Cortical Neuron Dysplasia, Cerebral Amyloid Angiopathy and TMA’s supporting the NIH National NeuroAIDS Tissue Consortium (NNTC). The facility provides support for large initiatives including the UCLA Lung and Prostate SPORE’s and serves as one of the validation laboratories for the NCI’s Early Detection Research Network (EDRN). Dr. Seligson directs TMA construction for the NCI National Lung Screening Trial (NLST) and the NCI Prostate, Lung, Colon, and Ovarian (PLCO) Cancer Screening Trial. The facility has also successfully collaborated with biopharmaceutical companies to validate putative therapeutic drug targets and biochemical pathways.

UCLA Tissue Array Core Facility-supported publications

Mumenthaler, S.M., Yu, H., Tze, S., Cederbaum, S.D., Pegg, A.E., Seligson, D.B., Grody, W.W., Expression of Arginase II in Prostate Cancer, Int. J. Oncol. 2008 Feb;32(2):357-65.

Seligson, D.B., Rajasekaran, S.A., Yu, H., Liu, X., Eeva, M., Tze, S., Ball, W., Horvath, S., deKernion, J.B., Rajasekaran, A.K., Na,K-Adenosine Triphosphatase Alpha(1)-Subunit Predicts Survival in Renal Clear Cell Carcinoma, J Urol. 2008 Jan;179(1):338-45.

Klatte, T., Seligson, D.B., Leppert, J.T., Riggs, S.B., Yu, H., Zomorodian, N., Kabbinavar, F.F., Strieter, R.M., Belldegrun, A.S., Pantuck, A.J., The Chemokine Receptor CXCR3 is an Independent Prognostic Factor in Patients With Localized Clear Cell Renal Cell Carcinoma, J Urol. 2008 Jan;179(1):61-6.

Seligson, D.B., Hongo, F., Huerta-Yepez, S., Mizutani, Y., Miki, T., Yu, H., Horvath, S., Chia, D., Goodglick, L., Bonavida, B., Expression of X-Linked Inhibitor of Apoptosis Protein is a Strong Predictor of Human Prostate Cancer Recurrence, Clin. Cancer Res. 2007 Oct 15;13(20):6056-6063

Mah, V., Seligson, D.B., Li, A., Márquez, D.C., Wistuba , I.I., Elshimali, Y., Fishbein, M.C., Chia, D., Pietras, R.J., Goodglick, L. Aromatase Expression Predicts Survival in Women with Early Stage Non Small Cell Lung Cancer, Cancer Res. 2007 Nov 1;67(21):10484-90

Klatte, T., Seligson, D.B., Riggs, S.B., Leppert, J.T., Berkman, M.K., Kleid, M.D., Yu, H., Kabbinavar, F.F., Pantuck, A.J., and Belldegrun, A.S., Hypoxia-Inducible Factor 1 alpha in Clear Cell Renal Cell Carcinoma: Protein Expression and Prognostic Impact, Clin Cancer Res. 2007 Dec 15;13(24):7388-93

Pantuck, A.J., Seligson, D.B., Klatte, T., Yu, H., Leppert, J.T., Moore, L., O'Toole, T., Gibbons, J., Belldegrun, A.S., Figlin, R.A., Prognostic Relevance of the mTOR Pathway in Renal Cell Carcinoma - Implications for Molecular Patient Selection for Targeted Therapy, Cancer 2007 Jun 1;109(11):2257-67.

Zhou, H., Miki, R., Eeva, M., Fike, F.M., Seligson, D., Yang, L., Yoshimura, A., Teitell, M.A., Jamieson, C.A., Cacalano, N.A. Reciprocal Regulation of SOCS 1 and SOCS3 Enhances Resistance to Ionizing Radiation in Glioblastoma Multiforme. Clin Cancer Res. 2007 Apr 15;13(8):2344-53.

Patel, A.A., Gupta, D., Seligson, D., Hattab, E.M., Balis, U.J., Ulbright, T.M., Kohane, I.S., Berman, J.J., Gilbertson, J.R., Dry, S., Schirripa, O., Yu, H., Becich, M.J., Parwani, A.V. Availability and Quality of Paraffin Blocks Identified in Pathology Archives: A Multi-Institutional Study by the Shared Pathology Informatics Network (SPIN). BMC Cancer 2007, Feb 28;7:37.

Weiss, R.H., Borowsky, A.D., Seligson, D.B., Lin, P.Y., Dillard-Telm, L., Belldegrun, A.S., Figlin, R.A. and Pantuck, A.J., p21 is a Prognostic Marker in Renal Cell Carcinoma: Implications for Novel Therapeutic Approaches, J Urol. 2007 Jan;177(1):63-8.

Shen, D., Nooraie, F., Elshimali, Y., Lonsberry, V., He, J., Bose, S., Chia, D., Seligson, D., Chang, H.R., Goodglick, L. Decreased Expression of Annexin A1 is Correlated with Breast Cancer Development and Progression as Determined by a Tissue Microarray Analysis. Hum Pathol. 2006 Dec;37(12):1583-91.

Tso, C.L., Shintaku, P., Chen, J., Liu, Q., Liu, J., Chen, Z., Yoshimoto, K., Mischel, P.S., Cloughesy, T.F., Liau, L.M., Nelson, S.F. Primary glioblastomas express mesenchymal stem-like properties. Mol Cancer Res. 2006 Sep;4(9):607-19.

Lam, J.S., Seligson, D.B., Yu, H., Li, A., Eeva, M., Pantuck, A.J., Zeng, G., Horvath, S., Belldegrun, A.S., Flap Endonuclease 1 is Overexpressed in Prostate Cancer and is Associated with a High Gleason Score. BJU Int. 2006 Aug;98(2):445-51.

Wadehra, M., Natarajan, S., Seligson, D.B., Williams, C., Braun, J., Soslow, R., Expression of Epithelial Membrane Protein-2 is Associated with Endometrial Adenocarcinoma of Unfavorable Outcome, Cancer 2006, May 30;107(1):90-98.

Seligson D.B., The Tissue Micro-array as a Translational Research Tool for Biomarker Profiling and Validation, Biomarkers, November 2005; 10 (Supplement 1): S77-S82.

Yang, X.J., Tan, M.H., Kim, H., Ditlev, J.A., Betten, M.W., Png, C.E., Kort, E.J., Futami, K., Dykema, K.J., Furge, K.A., Takahashi, M., Kanayama, H., Tan, P.H., Teh, B.S., Luan, C., Wang, K., Pins, M.R., Tretiakova, M., Anema, J., Kahnoski, R., Nicol, T., Stadler, W., Vogelzang, N.G., Amato, R., Seligson, D.B., Figlin, R., Belldegrun, A., Rogers, C.G., Teh, B.T., A Molecular Classification of Papillary Renal Cell Carcinoma, Cancer Res. 2005 Jul 1;65(13):5628-5637.

Lu KV, Jong KA, Kim GY, Singh J, Dia EQ, Yoshimoto K, Wang MY, Cloughesy TF, Nelson SF, Mischel PS. Differential Induction of Glioblastoma Migration and Growth by Two Forms of Pleiotrophin. J Biol Chem. 2005 Jul 22;280(29):26953-64.

Seligson, D.B., Horvath, S., Huerta, S., Hanna, S., Garban, H., Roberts, A., Shi, T., Liu, X., Chia, D., Goodglick, L., Bonavida, B., Expression of Transcription Factor Yin Yang 1 in Prostate Cancer, Int J Oncol. 2005 Jul;27(1):131-41.

Seligson, D.B., Horvath, S., Shi, T., Yu, H., Tze, S., Grunstein, M., Kurdistani, S., Global Histone Modification Patterns Predict Risk of Prostate Cancer Recurrence, Nature 2005 June 30;435:1262x1266.

Lam, J., Shvarts, O., Said, J., Pantuck, A., Seligson, D.B., Aldridge, M., Bui, M., Liu, X., Horvath, S., Figlin, R., Belldegrun, A., Clinicopathologic and Molecular Correlations of Necrosis in the Primary Tumor of Patients with Renal Cell Carcinoma, Cancer 2005 Jun 15;103(12):2517-25.

Kim, H.L., Seligson, D.B., Liu, X., Janzen, N., Bui, M.H.T, Yu, H., Shi, T., Belldegrun, A., Horvath, S., Figlin, R.A., Using Tumor Markers to Predict the Survival of Patients with Metastatic Renal Cell Carcinoma, J. of Urol. 2005 May;173(5):1496-501.

Shi, T., Seligson, D.B., Belldegrun, A., Palotie, A., Horvath, S., Tumor Classification by Tissue Microarray Profiling: Random Forest Clustering Applied to Renal Cell Carcinoma, Modern Pathology 2005 Apr;18(4):547-57.

Shvarts, O., Seligson, D, B., Lam, J., Shi, T., Horvath, S., Figlin, R., Belldegrun, A., Pantuck, A., P53 is an Independent Predictor of Tumor Recurrence and Progression after Nephrectomy in Patients with Localized Renal Cell Carcinoma, J. Urol. 2005 Mar;173(3):725-8.

Green D.A., Masliah E., Vinters H.V., Beizai P., Moore D.J., Achim C.L., Brain Deposition of Beta-Amyloid is a Common Pathologic Feature in HIV Positive Patients. AIDS. 2005 Mar 4;19(4):407-411.

Shen, D., Chang, H.R., Chen, Z., He, J., Lonsberry, V., Elshimali, Y., Chia, D., Seligson, D.B., Goodglick, L., Nelson, S.F., Gornbein, J., Loss Of Annexin A1 (ANXA1) Expression In Human Breast Cancer Detected By Multiple High-Throughput Analyses, Biochemical & Biophysical Research Communications, 2004; 326:218-227.

Liu, X., Minin, V., Huang, Y., Seligson, D.B., Horvath, S., Statistical Methods for Analyzing Tissue Microarray Data, J of Biopharm Stat. 2004; 14(3):671-685.

Miyata, H., Chiang, A.C., Vinters, H.V., Insulin Signaling Pathways in Cortical Dysplasia and TSC-Tubers: Tissue Microarray Analysis. Ann Neurol. 2004 Oct;56(4):510-9.

Kim, H.L., Seligson, D.B., Liu, X., Janzen, N., Bui, M.H.T, Yu, H., Shi, T., Figlin, R.A., Horvath, S., Belldegrun, A., Using Protein Expressions to Predict Survival in Clear Cell Renal Carcinoma, Clin Cancer Res. 2004 Aug 15;10(16):5464-71.

Garraway, I.P., Seligson, D.B., Said, J., Horvath, S., Reiter, R.E., Trefoil Factor 3 Is Overexpressed In Human Prostate Cancer, The Prostate 2004 9999:1-6.

Bui, M.H., Visapaa, H., Seligson, D.B., Kim, H., Han, K.R., Huang, Y., Horvath, S., Stanbridge, E.J., Palotie, A., Figlin, R.A., Belldegrun, A.S. Prognostic Value Of Carbonic Anhydrase IX And Ki67 As Predictors Of Survival For Renal Clear Cell Carcinoma, J Urol. 2004 Jun;171(6, Part 1 Of 2):2461-2466.

Zhang, L., Maul, R.S., Rao, J.Y., Apple, S., Seligson, D.B., Sartippour, M., Rubio, R., Brooks, M.N., Expression Pattern of the Novel Gene EG-1 in Cancer, Clin Cancer Res. 2004 May 15;10(10):3504-8.

Krysan, K., Dohadwala, M., Luo, J., Lin, Y., Zhu, L., Heuze-Vourc'h, N., Goodglick, L., Merchant, F., Seligson, D.B., Pold, M., Strieter, R., Sharma, S., Dubinett, S., Cyclooxygenase-2-Dependent Expression of Survivin in Non-small Cell Lung Cancer, Chest. 2004 May;125(5 Suppl):140S,

Seligson, D.B., Pantuck, A., Lui, X., Huang, Y., Horvath, S., Bui, M., Han, K.R., Correa, A.J.L., Eeva, M., Tze, S., Belldegrun, A., EpCAM (KSA) Expression: Pathobiology and its Role as an Independent Predictor of Survival in Renal Cell Carcinoma. Clin Cancer Res 2004 Apr 15;10(8):2659-69.

Han, K.R., Seligson, D.B., Liu, X., Horvath, S., Shintaku, P., Thomas, G.V., Said, J.W., Reiter, R.E., Prostate Stem Cell Antigen (PSCA) Expression is Associated with Gleason score, Seminal Vesicle Invasion, and Capsular Invasion in Prostate Cancer. J Urol 2004 Mar;171(3):1117-1121.

Vinters H.A., Klementa, I.A., Sunga, S.H., and Farag, E.S., Pathologic Issues and New Methodologies in the Evaluation of Non-Alzheimer Dementias, Clinical Neuroscience Research, Volume 3, Issue 6, March-April 2004, Pages 413-426.

Krysan, K., Merchant, F., Zhu L., Dohadwalaa, M., Luo, J., Lin, Y., Heuze-Vourc'h, N., Pold, M., Seligson, D.B., Chia, D., Goodglick, L., Strieter, R., Sharma, S., Dubinett, S., COX-Dependent Stabilization of Survivin in Non-Small Cell Lung Cancer. FASEB J. 2004 Jan; 18(1): 206-8.

Choe, G., Horvath, S., Cloughesy, T.,F., Crosby, K., Seligson, D.B., Palotie, A., Inge, L., Smith, B., Sawyers, C.,L. Mischel, P.S., Analysis of the Phosphatidylinositol 3x-Kinase Signaling Pathway in Glioblastoma Patients in Vivo. Cancer Res 2003 63: 2742-2746.

Mischel PS., Shai R., Choe G., Seligson, D.B., Kremen T, Palotie A., Liau L.M., Cloughesy T.F., Nelson S.F., Identification of Molecular Subtypes of Glioblastoma by Gene Expression Profiling, Oncogene 2003 Apr 17;22(15):2361-73.

Espineda, C., Seligson, D.B., Ball, W.J., Rao, J.Y., Palotie, A., Horvath, S., Huang, Y., Shi., T., and Rajasekaran, A., Analysis of the Na,K-ATPase alpha- and beta-Subunit Expression Profiles of Bladder Cancer Using Tissue Microarrays, Cancer 2003;97(8):1859-68.

Visapaa, H., Bui, M. Huang, Y., Seligson, D.B., Tsai, H., Pantuck, A., Figlin, R., Rao, J.Y., Belldegrun, A., Horvath, S., Palotie, A., Correlation of Ki-67 and Gelsolin Expression to Clinical Outcome in Renal Clear-Cell Carcinoma, Urology 61:845-850, 2003.

Freedland, S., Seligson, D.B., Liu, A.Y., Pantuck, A.J., Horvath, S., Wieder, J.A., Zisman, A., Nguyen, D., Tso, C.L., Palotie, A., and Belldegrun, A.S., Loss of CD10 (Neutral Endopeptidase) is a Frequent and Early event in Prostate Cancer, The Prostate 2003 55:71-80.

Visapaa, H., Seligson, D.B., Eeva, M., Gaber, F., Rao, J.Y., Belldegrun, A., Palotie, A., 8q24 Amplification in Transitional Cell Carcinoma of Bladder, Appl Immunohistochem Mol Morphol 2003 Mar;11(1):33-36.

Visapaa, H., Seligson, D.B., Huang, Y., Rao, J.Y., Belldegrun, A., Horvath, S., Palotie, A., Ki-67, Gelsolin and PTEN Expression in Sarcomatoid Renal Tumors, Urol Res 2003 Feb;30(6):387-9.

Bui, M., Seligson, D.B., Han, K., Pantuck, A., Dorey, F., Huang, Y., Horvath, S., Leibovich, B., Liao, S.Y., Stanbridge, E., Lerman,. M., Palotie,. A., deKernion, J.B., Figlin, R.A., Belldegrun, A., Carbonic Anhydrase IX is an Independent Predictor of Survival in Advanced Renal Cell Carcinoma: Implications for Prognosis and Therapy, Clinical Cancer Research 2003 Feb;9(2):802-811.

Ide, H., Seligson, D.B., Memarzadeh, S., Horvath, S., Xin, L., Raffii, B., Kacinski, B., Palotie, A., Witte, O.N., Expression of Colony Stimulating Factor 1 Receptor (CSF-1R) in Prostate Development and Prostate Cancer, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14404-14409.

Rao, J.Y., Seligson, D.B., Visapaa, H., Horvath, S., Eeva, M., Michel, K., Pantuck, A., Belldegrun, A., Palotie, A., Tissue Microarray (TMA) Analysis of Cytoskeletal Actin Associated Biomarkers Gelsolin and E-Cadherin in Urothelial Carcinoma, Cancer 2002 Sep 15;95(6):1247-57.

Goldstine J., Seligson D.B., Beizai P., Miyata H., Vinters H.V., Tissue Microarrays in the Study of Non-Neoplastic Disease of the Nervous System, J Neuropathology Exp Neurology, Aug;61(8): 653-662, 2002.

Rao, J.Y., Seligson, D.B., and Hemstreet, G., Protein Expression Analysis using Quantitative Fluorescence Image Analysis (QFIA) on Tissue Microarray Slides, Biotechniques 32(4) 924-933, 2002.